MAGUS Lum 400 Fluorescence Microscope

The microscope is designed for studying specimens using the fluorescence microscopy technique in reflected light and brightfield technique in transmitted light. The additional equipment allows for darkfield, polarization, and phase contrast microscopy. Fluorescence microscopy is based on the ability of some substances to glow when exposed to the light of a certain part of the spectrum. The specimens are exposed to invisible short-wave ultraviolet light or violet, blue, and green light. The wavelength of the emitted light will be longer than the wavelength of the excitation light. The specimen glows blue, cyan, green-yellow, or red light, respectively. Some specimens glow by themselves while others glow after treatment with fluorochromes. The fluorescent microscope is used to study pathogens of tuberculosis, chlamydia, rabies, herpes, and other diseases. The microscope can also be used for DNA and chromosome analysis, bone marrow and blood smears, forensic and pharmacological examination, veterinary control studies, and sanitary and epidemiological inspection.

Microscope head

Trinocular head with infinity-corrected optics. Eyepiece tubes can rotate 360°. The user can adjust eye relief on the eyepiece to fit their height. The digital camera is mounted in the trinocular tube.

Revolving nosepiece

Five objectives. A free slot is used for centering the reflected light source. An additional objective can also be installed in the free slot in order to achieve extra magnification. The revolving nosepiece with objectives is oriented toward the interior – the user can see the objective inserted into the optical path, and the space above the stage is free.

Objectives

Infinity plan achromatic objectives and infinity plan fluorite objectives. Standard configuration includes 4x, 10x, and 100x objectives for brightfield microscopy and a 40x objective for fluorescence microscopy.

Focusing mechanism

Coaxial coarse and fine focusing knobs are located at the base of the microscope on both sides. The user can place their hands on the table and take a relaxed pose while observing. The focusing adjustment is smooth and effortless. On the right, there is a coarse focusing lock knob for quick adjustments after changing the specimen. On the left, there is a coarse tension adjustment ring for further adjustment.

Stage

The stage does not have an X-axis positioning rack, which ensures its ergonomic operation. The mechanical stage with a belt-driven mechanism allows for the smooth movement of the object. The slide holder is fixed with two screws and can be removed for easy operation. For reflected light observations, a black stage plate should be placed on the stage to remove stray light.

Reflected light illuminator

The fluorescence excitation source is a 100W mercury lamp. It is extremely bright and has a wide spectrum of wavelengths. The mercury lamp has discrete peaks, allowing you to work with many fluorochromes. The reflected light illuminator contains four excitation filters: ultraviolet (UV), violet (V), blue (B), and green (G). The mercury lamp is located in the lamphouse and can be centered in three planes. The heat radiation is conducted away from the lamphouse so that the lamp does not overheat. There is a mount that allows for safe and quick lamp replacement.

Köhler illumination in reflected and transmitted light

Setting up the Köhler illumination enhances the image quality of a specimen. With such illumination, you can achieve maximum resolution on each objective and uniform illumination of the field of view with no darkening at the edges. The object of study is in sharp focus, and the image artifacts are removed.

Condenser

The condenser has a slot for a darkfield slider or a phase contrast slider. The usage of slider saves time when switching between microscopy techniques in transmitted light.

Transmitted light source

The transmitted light illuminator has a 30W halogen bulb. Halogen bulbs emit light with a color temperature that allows for comfortable work. The 30W bulb is bright enough to observe using 4x to 100x magnification objectives.

Accessories

There is a line of accessories designed for the microscope. Optional objectives provide additional magnification. The optional 10x/0.35 fluorite objective is used for capturing an image of a specimen with a resolution of 0.5μm over a large field of view. Eyepieces that extend the magnification range of the microscope. Optional eyepieces help you maximize the potential of the objective you use most often. A phase contrast device, a darkfield condenser, and a polarization device offer more microscopy techniques so that you can study the specimens that are invisible in brightfield or fluorescent light. A digital camera that outputs the microscope image on a monitor and stores files along with software that takes real-time measurements of specimens. A calibration slide for measuring objects that can be combined with an eyepiece with a scale or the camera software.

Key features:

• Study of specimens emitting fluorescence when exposed to excitation light
• Microscopy techniques: fluorescence in reflected light, brightfield in transmitted light
• Trinocular head with adjustable eye relief and a vertical tube for mounting a digital camera
• Reflected light illuminator: 100W mercury lamp of a wide wavelength spectrum
• Fluorescence filters: ultraviolet (UV), violet (V), blue (B), green (G)
• Transmitted light illuminator: 30W halogen bulb providing bright natural light
• Köhler illumination in reflected and transmitted light
• Simple stage without a positioning rack
• Wide range of compatible optional accessories

The kit includes:

• Base with a power input, transmitted light source and condenser, focusing mechanism, stage, and revolving nosepiece
• Reflected light illuminator
• Mercury lamphouse
• Trinocular head
• Infinity plan achromatic objective: PL 4x/0.10 WD 19.8mm
• Infinity plan achromatic objective: PL 10x/0.25 WD 5.0mm
• Infinity plan achromatic objective, fluo: PL FL 40x/0.85 (spring-loaded) WD 0.42mm
• Infinity plan achromatic objective, fluo: PL 100x/1.25 (spring-loaded, oil) WD 0.36mm
• Eyepiece 10x/22mm with long eye relief (2 pcs.)
• UV shield
• C-mount adapter 1x
• Hex key wrench
• Mercury lamphouse power supply
• Power cord
• Reflected light illuminator power cord
• Dust cover
• User manual and warranty card

Available on request:

• 10x/22mm eyepiece with a scale
• 12.5x/14mm eyepiece (2 pcs.)
• 15x/15mm eyepiece (2 pcs.)
• 20x/12mm eyepiece (2 pcs.)
• 25x/9mm eyepiece (2 pcs.)
• Infinity plan achromatic objective, fluo: PL FL 10x/0.35 WD 2.37mm
• Infinity plan achromatic objective: PL 60x/0.80 ∞/0.17 WD 0.46mm
• Phase contrast device
• Darkfield condenser
• Immersion darkfield condenser
• Darkfield slider
• Polarization device
• Digital camera
• Calibration slide