MAGUS Pol D800 Polarizing Digital Microscope

The microscope is designed for studying anisotropic geological, biological, and polymeric samples in polarized and transmitted light.
A polarizing microscope uses the birefringence of an anisotropic specimen to deliver an image. Plane-polarized light, when passing through an anisotropic specimen, splits into two beams and changes the plane of polarization. The analyzer brings the vibrations of the beams into the same plane and axis, thereby causing destructive and constructive interference to occur between the two wavefronts. The bright, high-contrast image changes color when the stage rotates.
This microscope has strain-free objectives. The intermediate tube holds the analyzer and Bertrand lens, and it has a slot for compensators. The microscope is used in crystallography, petrography, mineralogy, forensics, medicine, and other fields of science.

Digital camera

MAGUS CBF70 is a science camera with 21MP resolution and 4/3" sensor for the brightfield microscopy technique. The camera delivers a realistic image at a 5280x3954 pix resolution. The camera is recommended for use with 4x, 10x, 20x, and 40x objectives. When working with low magnification objectives, the camera will allow you to see finer details. At maximum resolution, video is captured at 17fps. It is enough for adjusting the focusing of the microscope. When the resolution is reduced, the rate increases to 56, 67, and 192fps. Transitions between frames become softer, the camera catches the slightest movement of the sample. These modes are suitable for classroom demonstrations, recording video of fast-moving processes, and observing samples that are moving. The camera is equipped with a USB 3.0 interface. The data transfer speed is 10 times faster than USB 2.0 cameras. The high-speed camera is recommended for professional laboratories, research, or university training.

Microscope head

Trinocular head with infinity plan achromatic objectives. Digital image capturing devices are mounted in the trinocular tube. The eyepiece/camera light path selection knob allows you to direct the light beam to either the digital camera or the eyepiece tubes. The diopter adjustment is on the left tube.

Revolving nosepiece

Five objectives. An additional objective can also be installed in a free slot in order to achieve extra magnification. The design of the revolving nosepiece (“away from the observer”) frees up space at the front of the stage so that the user can see the objective inserted into the optical path. The revolving nosepiece slots are centered to align the optical axis of the objective and microscope.

Objectives

Infinity plan achromatic objectives are designed specifically for polarized light observations: The strain-free optics ensure that the birefringence comes from the specimen and not from the optical elements. Designed for use with 0.17mm thick cover slips.

Focusing mechanism

Coaxial coarse and fine focusing knobs are located at the bottom of the stand on both sides. The user can place their hands on the table and take a relaxed pose while observing. The focusing adjustment is smooth and effortless.
The design of the focusing mechanism provides for quick microscope adjustment after changing the object of study. For this purpose, a coarse focusing lock knob is located on the right side. The coarse tension can be adjusted by turning the ring on the left side.

Stage

The stage rotates 360° to view the color change of the sample when the polarizer and analyzer are in crossed orientation. The stage has a gradation of the rotation angle. With the vernier scale, measurements are made with an accuracy of 0.1°.
The stage can be centered with two screws because the analysis of an anisotropic object in polarized light requires the precise alignment of the rotation axis of the stage with the optical axis of the microscope.

Light source

Halogen bulbs emit light with a color temperature that allows for comfortable work. The 30W bulb is bright enough for you to observe with the 4x to 100x magnification objectives that are used for brightfield and polarized light microscopy.

Transmitted light illumination

Köhler illumination: adjustable field diaphragm; centered and height-adjustable Abbe condenser with adjustable aperture diaphragm and swivel auxiliary lens. NA = 1.25. The polarizer is 0–360° rotatable, with four rotation angles of 0°, 90°, 180°, 270° marks on the scale. The analyzer rotates 90° and the vernier scale on the analyzer provides accurate angle measurement.

Köhler illumination

The Köhler illumination setup enhances the image quality of a specimen. With such illumination, you can achieve maximum resolution on each objective and the uniform illumination of the field of view with no darkening at the edges. The object of study is in sharp focus and the image artifacts are removed.

Studying samples in polarized light

The intermediate tube holds the analyzer and Bertrand lens, and it has a slot for compensators. The analyzer is inserted into the light path to observe objects in polarized light. When the polarizer and analyzer rotate, the polarization angle changes. You can achieve a mutually perpendicular position by setting both filters to the “0” position. When the microscope stand is rotated, depending on the polarization angle, the surface of the sample reflects light differently. The Bertrand lens is used for conoscopic studies. The compensators are designed to enhance the contrast of samples with weak birefringence.

Accessories

There is a line of accessories designed specifically for this microscope.
Additional objectives can be used for studying objects up to 0.22µm.
Eyepieces extend the magnification range of the microscope. Additional eyepieces will help you to utilize the full potential of an objective that is used more often.
A digital camera that outputs the microscope image on a monitor, stores files, and that has software that takes real-time measurements of specimens. A calibration slide for measuring objects that can be combined with an eyepiece with a scale or the camera software.

Key features of the microscope:

• Study of anisotropic samples in polarized and transmitted light
• Powerful 30W halogen transmitted light source that provides bright natural light
• Bertrand lens for conoscopy and orthoscopy, compensators, and Köhler illumination
• Trinocular head with a trinocular tube for mounting a digital camera; 100:0 and 0:100 beam splitting
• Plan achromatic strain-free objectives eliminate the false optic effects
• Convenient alignment of the revolving nosepiece slots and stage
• Rotatable round stage with the vernier scale for precise angle measurement
• Wide range of optional accessories

Key features of the camera:

• For brightfield microscopy observations with 4x, 10x, 20x, and 40x objectives
• 21MP resolution: When working with low magnification objectives, the camera will allow you to see finer details
• 17fps at maximum resolution is enough for focusing adjustment
• Up to 192fps at reduced resolution for classroom demonstrations, observation of moving samples, video recording; movement of a sample can be seen without jerkiness or delays
• SONY Exmor backlit color CMOS sensor provides low noise level and high light sensitivity even in low-light conditions. You will get clearer, brighter, and more color-saturated images
• USB 3.0 interface for fast, smooth data transfer
• Software with photo, video recording, editing, external display, and linear and angular measurement

The kit includes:

• MAGUS CBF70 Digital Camera (digital camera, USB cable, installation CD with drivers and software, user manual and warranty card)
• Base with a power input, transmitted light source and condenser, focusing mechanism, stage, and revolving nosepiece
• Trinocular head
• Intermediate tube with Bertrand lens, an analyzer, and a compensator slot
• Infinity plan achromatic objective: PL 4x/0.10 WD 21mm
• Infinity plan achromatic objective: PL 10x/0.25 WD 5.0mm
• Infinity plan achromatic objective: PL 40x/0.65 (spring-loaded) WD 0.66mm
• Infinity plan achromatic objective: PL 60x/0.80 (spring-loaded) WD 0.46mm
• Eyepiece 10x/22mm with long eye relief (2 pcs.)
• 10x/22mm eyepiece with a scale. Scale value: 0.1mm
• C-mount adapter 1x
• Hex key wrench
• Power cord
• Dust cover
• User manual and warranty card

Available on request:

• Eyepiece 10x/22mm with crosshairs
• 12.5x/14mm eyepiece (2 pcs.)
• 15x/15mm eyepiece (2 pcs.)
• 20x/12mm eyepiece (2 pcs.)
• 25x/9mm eyepiece (2 pcs.)
• Infinity plan achromatic objective: PL 5x/0.12 WD 26.1mm
• Infinity plan achromatic objective: PL 20x/0.4 WD 8.8mm
• Infinity plan achromatic objective, fluo: PL 100x/1.25 (spring-loaded, oil) WD 0.36mm
• Infinity plan achromatic objective, fluo: PL 100x/0.8 (spring-loaded, dry) WD 2.02mm
• Mechanical stage attachment
• Calibration slide